Event Details
Label-Free Study of Bovine Serum Albumin Conformational Dynamics & Electrostatic Interaction between Proteins and Gold Surface using Optical Nanotweezers
Presenter: Sherin George
Supervisor:
Date: Mon, November 18, 2024
Time: 11:30:00 - 00:00:00
Place: Zoom, link below.
ABSTRACT
Abstract:
Single molecule analysis of proteins in an aqueous environment without modification (e.g., labels or tethers) elucidates their biophysics and interactions relevant to drug discovery. Forces of radiation pressure from a laser beam can be used to accelerate and trap freely suspended particles as discovered by Ashkin in the 1970s. Optical traps are formed by tightly focusing a laser beam using a high numerical aperture objective lens. The conformational dynamics of a single unmodified protein have been largely unexplored, and this study intends to investigate these dynamics in a single unmodified BSA protein utilizing DNH optical tweezers. In addition , the electrostatic interactions of different unmodified proteins with gold surface is studied using DNH optical tweezers to explore its influence on the trapping efficiency.
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